OpenPlant PI Nicola Patron and Oleg Raitskin have published a free to read editorial in Current Opinion in Biotechnology on ‘Multi-gene engineering in plants with RNA-guided Cas9 nuclease’
- RNA-guided Cas9 nucleases have been employed for genome editing in several plant species.
- Several strategies for simultaneous expression of multiple sgRNAs have been demonstrated.
- Parallel DNA assembly methods enable rapid construction of vectors for plant genome editing.
For more information see the full article.